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kallisto | bustools

基于伪比对的快速单细胞 RNA-seq 预处理流程,利用 kallisto 索引和 bustools 流式处理实现轻量级定量。 该方法内存占用极低,处理速度快,支持多种单细胞平台,是大规模单细胞数据预处理的高效替代方案。

PropertyValue
Purpose快速轻量的单细胞 RNA-seq 数据预处理
Time ComplexityO(n * k)
Space ComplexityO(g)
Year2021
DifficultyIntermediate
LanguagesC++
CategorySingle-Cell Genomics

Complexity Analysis

  • Time Complexity: O(n * k)
  • Space Complexity: O(g)

Performance Insight: The time complexity of this algorithm is polynomial.

Note: Complexity analysis is based on theoretical models. Actual runtime is affected by input scale, hardware, and implementation optimizations. Benchmark for your specific workload.

Literature & Implementation

Cell Ranger · STARsolo · Alevin

Tags

preprocessing pseudoalignment fast lightweight

Released under the MIT License.